Recombinant humaan Lyn-eiwit

Tyrosine-protein kinase Lyn, also known as LYN, is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. This protein is Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. It Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents. Recombinant human LYN protein, fused to His-tag at N-terminus, was expressed in E. coli.

Tyrosine-protein kinase Lyn, JTK8, p53Lyn, p56Lyn

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

61.0 kDa (535aa)

Lyn, Tyrosine-protein kinase Lyn, JTK8, p53Lyn, p56Lyn, ATGP1918-10 µg, ATGP1918-20 µg, ATGP1918-50 µg, ATGP1918-100 µg, ATGP1918-250 µg, ATGP1918-500 µg, ATGP1918-1 mg, ATGP1918-10, ATGP1918-20, ATGP1918-50, ATGP1918-100, ATGP1918-250, ATGP1918-500, ATGP1918-1

Gaul B.S., et al. (2001) J. Biol. Chem. Res. 275:16174-16182; ; Rena G., et al. (2001) Mol. Pharmacol. 59:996-1011

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Signal Transduction

NP_002341

P07948

Human

MGCIKSK GKDSLSDDGV DLKTQPVRNT ERTIYVRDPT SNKQQRPVPE SQLLPGQRFQ TKDPEEQGDI VVALYPYDGI HPDDLSFKKG EKMKVLEEHG EWWKAKSLLT KKEGFIPSNY VAKLNTLETE EWFFKDITRK DAERQLLAPG NSAGAFLIRE SETLKGSFSL SVRDFDPVHG DVIKHYKIRS LDNGGYYISP RITFPCISDM IKHYQKQADG LCRRLEKACI SPKPQKPWDK DAWEIPRESI KLVKRLGAGQ FGEVWMGYYN NSTKVAVKTL KPGTMSVQAF LEEANLMKTL QHDKLVRLYA VVTREEPIYI ITEYMAKGSL LDFLKSDEGG KVLLPKLIDF SAQIAEGMAY IERKNYIHRD LRAANVLVSE SLMCKIADFG LARVIEDNEY TAREGAKFPI KWTAPEAINF GCFTIKSDVW SFGILLYEIV TYGKIPYPGR TNADVMTALS QGYRMPRVEN CPDELYDIMK MCWKEKAEER PTFDYLQSVL DDFYTATEGQ YQQQP