Recombinant humaan LYPLA2-eiwit

Acyl-protein thioesterase 2, also known LYPLA2, is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein may hydrolyze fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Recombinant human LYPLA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Acyl-protein thioesterase 2, APT-2, APT2, Lysophospholipase II, LPL-II, LysoPLA II

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

26.9 kDa (251aa) confirmed by MALDI-TOF

LYPLA2, Acyl-protein thioesterase 2, APT-2, APT2, Lysophospholipase II, LPL-II, LysoPLA II, ATGP0926-10 µg, ATGP0926-20 µg, ATGP0926-50 µg, ATGP0926-100 µg, ATGP0926-250 µg, ATGP0926-500 µg, ATGP0926-1 mg, ATGP0926-10, ATGP0926-20, ATGP0926-50, ATGP0926-100, ATGP0926-250, ATGP0926-500, ATGP0926-1

Fan J., et al. (2008) Int J Infect Dis. 12 (2) :143-51.; ; Wanq A., et al. (2000) Biochim Biophys Acta. 1484:207-14.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol,100 mM NaCl

Metabolism

NP_009191.1

O95372

Human

MCGNTMSVPL LTDAATVSGA ERETAAVIFL HGLGDTGHSW ADALSTIRLP HVKYICPHAP RIPVTLNMKM VMPSWFDLMG LSPDAPEDEA GIKKAAENIK ALIEHEMKNG IPANRIVLGG FSQGGALSLY TALTCPHPLA GIVALSCWLP LHRAFPQAAN GSAKDLAILQ CHGELDPMVP VRFGALTAEK LRSVVTPARV QFKTYPGVMH SSCPQEMAAV KEFLEKLLPP V