Recombinant humaan M-CSF-eiwit

Macrophage colony stimulating factor (M-CSF), as known as CSF-1, one of the hematopoietic growth factors that regulate the growth and differentiation of blood cells. This protein is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osteoclasts. Recombinant human M-CSF, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer..

Macrophage colony stimulating factor, CSF1, Macrophage colony stimulating factor Colony stimulating factor 1, CSF 1, Lanimostim, M CSF, Macrophage Colony Stimulating Factor 1, MCSF, MGC31930.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

20.7 kDa (179aa) confirmed by MALDI-TOF

M-CSF, Macrophage colony stimulating factor, CSF1, Macrophage colony stimulating factor Colony stimulating factor 1, CSF 1, Lanimostim, M CSF, Macrophage Colony Stimulating Factor 1, MCSF, MGC31930., ATGP0432-10 µg, ATGP0432-20 µg, ATGP0432-50 µg, ATGP0432-100 µg, ATGP0432-250 µg, ATGP0432-500 µg, ATGP0432-1 mg, ATGP0432-10, ATGP0432-20, ATGP0432-50, ATGP0432-100, ATGP0432-250, ATGP0432-500, ATGP0432-1

Wei S., et al. (2006) . J Leukoc Biol. 80 (6) :1445-53.; ; Woo KM., et al. (2002) . Exp Mol Med. 34 (5) :340-6.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) 2mM DTT, 10% glycerol

Hormones & Cytokines

NP_757351.2

P09603

Human

EEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ DVVTKPDCN