Recombinant humaan M-CSF R/CD115 eiwit

CSF1R, also known as macrophage colony-stimulating factor 1 receptor, is a member of the type 3 subfamily of receptor tyrosine kinases. It is expressed primarily on cells of the monocyte and macrophage lineage, stem cells, and in the developing placenta and mediates most of the biological effects of this cytokine. This protein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. CSF1 and this receptor were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role of CSF1/CSF1R in normal mammary gland development is very intriguing because this relationship has also been found in the biology of breast cancer in which abnormal expression of CSF1 and its receptor. Also, increased levels of CSF1R are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Recombinant human CSF1R, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured by its ability to inhibit proliferation using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤100 ng/mL with Human M-CSF.

Macrophage colony-stimulating factor 1 receptor, CSF1R, C-FMS, CD115, CSF-1R, CSFR, FIM2, FMS, HDLS, M-CSF-R

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its ability to inhibit proliferation using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤100 ng/mL with Human M-CSF.

82.1 kDa (737aa)

M-CSF CD115, ATGP3794-10 µg, ATGP3794-20 µg, ATGP3794-50 µg, ATGP3794-100 µg, ATGP3794-250 µg, ATGP3794-500 µg, ATGP3794-1 mg, ATGP3794-10, ATGP3794-20, ATGP3794-50, ATGP3794-100, ATGP3794-250, ATGP3794-500, ATGP3794-1

Yeung YG., et al, (1998) J Biol Chem. 273:17128-17137.; ; Sapi E., et al, (1999) Proc Soc Exp Biol Med. 220:1-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Angiogenesis

NP_005202.2

P07333

Human

Ile20