Recombinant humaan MAD2L1-eiwit

MAD2L1, also known as MAD2L1-binding protein, is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Recombinant human MAD2L1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

MAD2L1-binding protein, HSMAD2, MAD2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25.7 kDa (225aa) confirmed by MALDI-TOF

MAD2L1, MAD2L1-binding protein, HSMAD2, MAD2, ATGP1216-10 µg, ATGP1216-20 µg, ATGP1216-50 µg, ATGP1216-100 µg, ATGP1216-250 µg, ATGP1216-500 µg, ATGP1216-1 mg, ATGP1216-10, ATGP1216-20, ATGP1216-50, ATGP1216-100, ATGP1216-250, ATGP1216-500, ATGP1216-1

Habu T., et al. (2002) EMBO J. 21:6419-6428; ; Matsuoka S., et al. (2007) Science. 316:1160-1166

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Cell Cycle

NP_002349.1

Q13257

Human

MALQLSREQG ITLRGSAEIV AEFFSFGINS ILYQRGIYPS ETFTRVQKYG LTLLVTTDLE LIKYLNNVVE QLKDWLYKCS VQKLVVVISN IESGEVLERW QFDIECDKTA KDDSAPREKS QKAIQDEIRS VIRQITATVT FLPLLEVSCS FDLLIYTDKD LVVPEKWEES GPQFITNSEE VRLRSFTTTI HKVNSMVAYK IPVND