Recombinant humaan MafG-eiwit

MAFG, also known as transcription factor MafG, belongs to the Maf family. Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Recombinant human MAFG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Transcription factor MafG, hMAF, MAFG, V-maf musculoaponeurotic fibrosarcoma oncogene homolog G

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by BCA assay)

> 85% by SDS-PAGE

20 kDa (182aa) confirmed by MALDI-TOF

MafG, Transcription factor MafG, hMAF, ATGP1268-10 µg, ATGP1268-20 µg, ATGP1268-50 µg, ATGP1268-100 µg, ATGP1268-250 µg, ATGP1268-500 µg, ATGP1268-1 mg, ATGP1268-10, ATGP1268-20, ATGP1268-50, ATGP1268-100, ATGP1268-250, ATGP1268-500, ATGP1268-1

Hung H.-L., et al. (2001) J. Biol. Chem. 276:10715-10721; ; Blank V., et al. (1997) Genomics. 44:147-149

Lacher SE, et al. Beyond antioxidant genes in the ancient NRF2 regulatory network. (Free Radic Biol Med. 2015) {https://pubmed.ncbi.nlm.nih.gov/26163000/}; ; Lacher SE, et al. A hypermorphic antioxidant response element is associated with increased MS4A6A expression and Alzheimers disease. (Redox Biol. 2018) {https://pubmed.ncbi.nlm.nih.gov/29179108/}; ; Lacher SE, et al. An antioxidant response element regulates the HIF1α axis in breast cancer cells. (Free Radic Biol Med. 2023) {https://pubmed.ncbi.nlm.nih.gov/37179033/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl

Epigenomics (Transcription & Translation)

NP_116100.2

O15525

Human

MTTPNKGNKA LKVKREPGEN GTSLTDEELV TMSVRELNQH LRGLSKEEIV QLKQRRRTLK NRGYAASCRV KRVTQKEELE KQKAELQQEV EKLASENASM KLELDALRSK YEALQTFART VARSPVAPAR GPLAAGLGPL VPGKVAATSV ITIVKSKTDA RS