Recombinant humaan MafK-eiwit

MAFK belongs to the bZIP family and Maf subfamily. Members of the Maf family of basic region/leucine zipper (bZIP) transcription factors affect transcription in either a positive or negative fashion, depending on their particular protein partner and the context of the target promoter. Small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Recombinant human MAFK protein, fused to His-tag at N-terminus, was expressed in E. coli.

Transcription factor MafK, NFE2u, P18

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

19.7 kDa (176aa)

MafK, Transcription factor MafK, NFE2u, P18, ATGP1931-10 µg, ATGP1931-20 µg, ATGP1931-50 µg, ATGP1931-100 µg, ATGP1931-250 µg, ATGP1931-500 µg, ATGP1931-1 mg, ATGP1931-10, ATGP1931-20, ATGP1931-50, ATGP1931-100, ATGP1931-250, ATGP1931-500, ATGP1931-1

Iwata T, et al. (1998) Cytogenet Cell Genet 82 (1-2) : 88-90.; ; Du MJ, et al. (2008) Int J Biochem Cell Biol. 40 (8) :1481-93.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Epigenomics (Transcription & Translation)

NP_002351

O60675

Human

MGSSHHHHHH SSGLVPRGSH MTTNPKPNKA LKVKKEAGEN APVLSDDELV SMSVRELNQH LRGLTKEEVT RLKQRRRTLK NRGYAASCRI KRVTQKEELE RQRVELQQEV EKLARENSSM RLELDALRSK YEALQTFART VARGPVAPSK VATTSVITIV KSTELSSTSV PFSAAS