Recombinant humaan MAGEA4-eiwit

MAGEA4 is a member of the MAGEA family, which directs the expression of tumor antigens that are recognized on a human melanoma by autologous cytolytic T lymphocytes (CTL) . The function of MAGEA4 is not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression. This protein is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Recombinant human MAGEA4, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Melanoma-associated antigen 4, Melanoma-associated antigen 4, Cancer/testis antigen 1.4, CT1.4, MAGE 4, MAGE 4 antigen, MAGE 41, MAGE 41 antigen, MAGE 4A, MAGE 4B, MAGE X2, MAGE X2 antigen, MAGE41, MAGE4A, MAGE4B, MAGEA 4, MAGEA4, Melanoma antigen family A 4

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37 kDa (337aa) confirmed by MALDI-TOF

MAGEA4, ATGP0594-10 µg, ATGP0594-20 µg, ATGP0594-50 µg, ATGP0594-100 µg, ATGP0594-250 µg, ATGP0594-500 µg, ATGP0594-1 mg, ATGP0594-10, ATGP0594-20, ATGP0594-50, ATGP0594-100, ATGP0594-250, ATGP0594-500, ATGP0594-1

Fradet Y., et al. (2009) Int J Cancer. 125 (6) :1365-71.; ; Nishimura T., et al. (2009) Br J Cancer. 100 (7) :1135-43.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Cancer

AAH17723.1

P43358

Human

MSSEQKSQHC KPEEGVEAQE EALGLVGAQA PTTEEQEAAV SSSSPLVPGT LEEVPAAESA GPPQSPQGAS ALPTTISFTC WRQPNEGSSS QEEEGPSTSP DAESLFREAL SNKVDELAHF LLRKYRAKEL VTKAEMLERV IKNYKRCFPV IFGKASESLK MIFGIDVKEV DPTSNTYTLV TCLGLSYDGL LGNNQIFPKT GLLIIVLGTI AMEGDSASEE EIWEELGVMG VYDGREHTVY GEPRKLLTQD WVQENYLEYR QVPGSNPARY EFLWGPRALA ETSYVKVLEH VVRVNARVRI AYPSLREAAL LEEEEGV