Recombinant humaan MAGEA5 eiwit

MAGEA5, also known as melanoma-associated antigen 5, is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This MAGEA gene encodes a protein that is C-terminally truncated compared to other family members, and this gene can be alternatively interpreted to be a pseudogene. Recombinant human MAGEA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Melanoma-associated antigen 5, CT1.5, MAGE5, MAGEA4

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

15.6 kDa (148aa) confirmed by MALDI-TOF, (Molecular weight on SDS-PAGE will appear higher)

MAGEA5, Melanoma-associated antigen 5, CT1.5, MAGE5, MAGEA4, ATGP1477-10 µg, ATGP1477-20 µg, ATGP1477-50 µg, ATGP1477-100 µg, ATGP1477-250 µg, ATGP1477-500 µg, ATGP1477-1 mg, ATGP1477-10, ATGP1477-20, ATGP1477-50, ATGP1477-100, ATGP1477-250, ATGP1477-500, ATGP1477-1

De Plaen E., et al. (1994) Immunogenetics. 40:360-369; ; The MGC Project Team. (2004) Genome Res. 14:2121-2127

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1mM PMSF

Cancer

NP_066387

P43359

Human

MSLEQK SQHCKPEEGL DTQEEALGLV GVQAATTEEQ EAVSSSSPLV PGTLGEVPAA GSPGPLKSPQ GASAIPTAID FTLWRQSIKG SSNQEEEGPS TSPDPESVFR AALSKKVADL IHFLLLKY