Recombinant humaan MAKKAPK3-eiwit

MAP kinase-activated protein kinase 3, also known as MAPKAPK3, is involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating Au-rich elements (AREs) -binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Recombinant human MAPKAPK3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

MAP kinase-activated protein kinase 3, 3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MK-3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

45.4 kDa (405aa) confirmed by MALDI-TOF

MAPKAPK3, MAP kinase-activated protein kinase 3, 3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MK-3, ATGP1805-10 µg, ATGP1805-20 µg, ATGP1805-50 µg, ATGP1805-100 µg, ATGP1805-250 µg, ATGP1805-500 µg, ATGP1805-1 mg, ATGP1805-10, ATGP1805-20, ATGP1805-50, ATGP1805-100, ATGP1805-250, ATGP1805-500, ATGP1805-1

Voncken J.W., et al. (2005) J. Biol. Chem. 280:5178-5187; ; Mendoza H., et al. (2008) Biochem. J. 409:711-722;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_004626

Q16644

Human

MGSSHHHHHH SSGLVPRGSH MGSMDGETAE EQGGPVPPPV APGGPGLGGA PGGRREPKKY AVTDDYQLSK QVLGLGVNGK VLECFHRRTG QKCALKLLYD SPKARQEVDH HWQASGGPHI VCILDVYENM HHGKRCLLII MECMEGGELF SRIQERGDQA FTEREAAEIM RDIGTAIQFL HSHNIAHRDV KPENLLYTSK EKDAVLKLTD FGFAKETTQN ALQTPCYTPY YVAPEVLGPE KYDKSCDMWS LGVIMYILLC GFPPFYSNTG QAISPGMKRR IRLGQYGFPN PEWSEVSEDA KQLIRLLLKT DPTERLTITQ FMNHPWINQS MVVPQTPLHT ARVLQEDKDH WDEVKEEMTS ALATMRVDYD QVKIKDLKTS NNRLLNKRRK KQAGSSSASQ GCNNQ