Recombinant humaan Malaatdehydrogenase 2/MDH2-eiwit

MDH2 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant human MDH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 700 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Malate dehydrogenase 2 NAD (mitochondrial), M-MDH, MDH, MOR1

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 700unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

35.2 kDa (335aa) confirmed by MALDI-TOF

MDH2, Malate dehydrogenase 2 NAD (mitochondrial), M-MDH, MDH, MOR1, Malate dehydrogenase 2, NAD (mitochondrial), ATGP0679-10 µg, ATGP0679-20 µg, ATGP0679-50 µg, ATGP0679-100 µg, ATGP0679-250 µg, ATGP0679-500 µg, ATGP0679-1 mg, ATGP0679-10, ATGP0679-20, ATGP0679-50, ATGP0679-100, ATGP0679-250, ATGP0679-500, ATGP0679-1

Thummel CS., et al. (2010) Dev Dyn. 239 (3) :954-64; ; Lee JS., et al (2002) J Basic Microbiol. 42 (4) :238-45.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Signal Transduction

NP_005909

P40926

Human

AKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK