Recombinant humaan MANF-eiwit

Mesencephalic astrocyte-derived neurotrophic factor, also known as MANF, is a 20 kDa member of the ARMET family of proteins. MANF was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress. Recombinant human MANF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Mesencephalic astrocyte-derived neurotrophic factor, ARMET, ARP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

20.8 kDa (183aa) confirmed by MALDI-TOF

MANF, Mesencephalic astrocyte-derived neurotrophic factor, ARMET, ARP, ATGP1465-10 µg, ATGP1465-20 µg, ATGP1465-50 µg, ATGP1465-100 µg, ATGP1465-250 µg, ATGP1465-500 µg, ATGP1465-1 mg, ATGP1465-10, ATGP1465-20, ATGP1465-50, ATGP1465-100, ATGP1465-250, ATGP1465-500, ATGP1465-1

Shridhar V., et al. (1996) Cancer Res. 56:5576.; ; Petrova P S., et al. (2003) J Mol Neurosci. 20:173.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Neuroscience

NP_006001

P55145

Human

LRPGD CEVCISYLGR FYQDLKDRDV TFSPATIENE LIKFCREARG KENRLCYYIG ATDDAATKII NEVSKPLAHH IPVEKICEKL KKKDSQICEL KYDKQIDLST VDLKKLRVKE LKKILDDWGE TCKGCAEKSD YIRKINELMP KYAPKAASAR TDL