Recombinant humaan MAPRE1-eiwit

MAPRE1, also known as EB1, belongs to the intermediate/early gene family. This protein first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. It localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. MAPRE1 is involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. Recombinant human MAPRE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Microtubule-associated protein RP/EB family member 1., Microtubule-associated protein RP/EB family member 1., EB1, MGC117374, MGC129946

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

32.1 kDa (288aa) confirmed by MALDI-TOF

MAPRE1, Microtubule-associated protein RP/EB family member 1., Microtubule-associated protein RP/EB family member 1., EB1, MGC117374, MGC129946, ATGP0851-10 µg, ATGP0851-20 µg, ATGP0851-50 µg, ATGP0851-100 µg, ATGP0851-250 µg, ATGP0851-500 µg, ATGP0851-1 mg, ATGP0851-10, ATGP0851-20, ATGP0851-50, ATGP0851-100, ATGP0851-250, ATGP0851-500, ATGP0851-1

Zhang T., et al. (2009) J Cell Sci. 122 (Pt 9) :1401-9. ; ; Tirnauer JS., et al. (2002) Mol Biol Cell. 13 (10) :3614-26.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cytoskeleton

NP_036457

Q15691

Human

MAVNVYSTSV TSDNLSRHDM LAWINESLQL NLTKIEQLCS GAAYCQFMDM LFPGSIALKK VKFQAKLEHE YIQNFKILQA GFKRMGVDKI IPVDKLVKGK FQDNFEFVQW FKKFFDANYD GKDYDPVAAR QGQETAVAPS LVAPALNKPK KPLTSSSAAP QRPISTQRTA AAPKAGPGVV RKNPGVGNGD DEAAELMQQV NVLKLTVEDL EKERDFYFGK LRNIELICQE NEGENDPVLQ RIVDILYATD EGFVIPDEGG PQEEQEEY