Recombinant humaan MAPRE2-eiwit

MAPRE2, also known as RP1 or EB2, is evolutionarily conserved proteins, which associate with the tips of growing microtubules, and regulate microtubule dynamics and their interactions with intracellular structures. It has been shown that EB proteins regulate microtubule function through CLIP proteins. Recombinant human MAPRE2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Microtubule-associated protein RP/EB family member 2, Microtubule-associated protein, RP/EB family member 2, EB1, EB2, RP1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

39.1 kDa (347aa) confirmed by MALDI-TOF

MAPRE2, Microtubule-associated protein RP/EB family member 2, Microtubule-associated protein, RP/EB family member 2, EB1, EB2, RP1, ATGP1213-10 µg, ATGP1213-20 µg, ATGP1213-50 µg, ATGP1213-100 µg, ATGP1213-250 µg, ATGP1213-500 µg, ATGP1213-1 mg, ATGP1213-10, ATGP1213-20, ATGP1213-50, ATGP1213-100, ATGP1213-250, ATGP1213-500, ATGP1213-1

Renner C., et al. (1997) J. Immunol. 159:1279-1283; ; Bu W., et al. (2003) J. Biol. Chem. 278:49721-49731

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Cytoskeleton

NP_055083.1

Q15555

Human

MPGPTQTLSP NGENNNDIIQ DNNGTIIPFR KHTVRGERSY SWGMAVNVYS TSITQETMSR HDIIAWVNDI VSLNYTKVEQ LCSGAAYCQF MDMLFPGCIS LKKVKFQAKL EHEYIHNFKL LQASFKRMNV DKVIPVEKLV KGRFQDNLDF IQWFKKFYDA NYDGKEYDPV EARQGQDAIP PPDPGEQIFN LPKKSHHANS PTAGAAKSSP AAKPGSTPSR PSSAKRASSS GSASKSDKDL ETQVIQLNEQ VHSLKLALEG VEKERDFYFG KLREIELLCQ EHGQENDDLV QRLMDILYAS EEHEGHTEEP EAEEQAHEQQ PPQQEEY