Recombinant humaan MASA/ENOPH1-eiwit

Enolase-phosphatase E1, also known as ENOPH1, is a member of the MasA family of the HAD (halo-acid dehalogenase) -like hydrolase superfamily. ENOPH1 is a bifunctional enzyme, exhibiting both phosphatase and atypical enolase activities. ENOPH1 plays an important role in the ubiquitous methionine salvage pathway, a biochemical pathway found in all organisms that regulate methionine levels in the cell. Recombinant human ENOPH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Enolase-phosphatase E1, MASA, MST145, Acireductone synthase, 2,3-diketo-5-methylthio-1-phosphopentane phosphatase, E1, mtnC

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31 kDa (281aa) confirmed by MALDI-TOF

ENOPH1, Enolase-phosphatase E1, MASA, MST145, ATGP0927-10 µg, ATGP0927-20 µg, ATGP0927-50 µg, ATGP0927-100 µg, ATGP0927-250 µg, ATGP0927-500 µg, ATGP0927-1 mg, ATGP0927-10, ATGP0927-20, ATGP0927-50, ATGP0927-100, ATGP0927-250, ATGP0927-500, ATGP0927-1

Zhang Y., et al. (2004) Bioorq Med Chem. 12:3847-3855.; ; Wang H., et al. (2005) Acta Crystallogr. 61:521-523.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Metabolism

NP_067027

Q9UHY7

Human

MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE ECQQDVSLLR KQAEEDAHLD GAVPIPAASG NGVDDLQQMI QAVVDNVCWQ MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY IYSSGSVEAQ KLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLIT SFSELYLPSS T