Recombinant humaan MAT1A-eiwit

MAT1A catalyzes the formation of S-adenosyltransferase (AdoMet) for methionine catabolism in the liver. MAT1A expression also correlates with a differentiated phenotype, whereas liver cells expressing MAT2A present a dedifferentiated phenotype and lowered AdoMet synthesis. Likewise, NFkappaB and TNFalpha cause a switch from MAT1A to MAT2A expression in human hepatocellular carcinoma (HCC), which facilitates cancer cell growth. Recombinant human MAT1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

S-adenosylmethionine synthetase, MAT, MATA1, SAMS, SAMS1, methionine adenosyltransferase I alpha, S-adenosylmethionine synthetase, MAT1A

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

45.8 kDa (415aa) confirmed by MALDI-TOF

MAT1A, S-adenosylmethionine synthetase, MAT, MATA1, SAMS, SAMS1, methionine adenosyltransferase I alpha, S-adenosylmethionine synthetase, MAT1A, ATGP0489-10 µg, ATGP0489-20 µg, ATGP0489-50 µg, ATGP0489-100 µg, ATGP0489-250 µg, ATGP0489-500 µg, ATGP0489-1 mg, ATGP0489-10, ATGP0489-20, ATGP0489-50, ATGP0489-100, ATGP0489-250, ATGP0489-500, ATGP0489-1

Ding W., et al. (2009) Hepatology. 49 (4) :1277-86. ; ; Tomasi ML., et al. (2009) Gastroenterology. 136 (3) :1025-36.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol

Metabolism

NP_000420.1

Q00266

Human

MNGPVDGLCD HSLSEGVFMF TSESVGEGHP DKICDQISDA VLDAHLKQDP NAKVACETVC KTGMVLLCGE ITSMAMVDYQ RVVRDTIKHI GYDDSAKGFD FKTCNVLVAL EQQSPDIAQC VHLDRNEEDV GAGDQGLMFG YATDETEECM PLTIILAHKL NARMADLRRS GLLPWLRPDS KTQVTVQYMQ DNGAVIPVRI HTIVISVQHN EDITLEEMRR ALKEQVIRAV VPAKYLDEDT VYHLQPSGRF VIGGPQGDAG VTGRKIIVDT YGGWGAHGGG AFSGKDYTKV DRSAAYAARW VAKSLVKAGL CRRVLVQVSY AIGVAEPLSI SIFTYGTSQK TERELLDVVH KNFDLRPGVI VRDLDLKKPI YQKTACYGHF GRSEFPWEVP RKLVF