Recombinant humaan MCL1-eiwit

MCL1, also known as BCL2L3, belongs to the Bcl-2 family. MCL1 is involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. Recombinant human MCL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Induced myeloid leukemia cell differentiation protein Mcl-1, BCL2L3, mcl1/EAT, myeloid cell leukemia sequence 1, Bcl-2-like protein 3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.2 kDa (347aa) confirmed by MALDI-TOF

MCL1, ATGP1696-10 µg, ATGP1696-20 µg, ATGP1696-50 µg, ATGP1696-100 µg, ATGP1696-250 µg, ATGP1696-500 µg, ATGP1696-1 mg, ATGP1696-10, ATGP1696-20, ATGP1696-50, ATGP1696-100, ATGP1696-250, ATGP1696-500, ATGP1696-1

Bingle C.D., et al. (2000) J. Biol. Chem. 275:22136-22146; ; Bae J., et al. (2000) J. Biol. Chem. 275:25255-25261

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT

Metabolism

NP_068779.1

Q07820

Human

MFGLKRNAVI GLNLYCGGAG LGAGSGGATR PGGRLLATEK EASARREIGG GEAGAVIGGS AGASPPSTLT PDSRRVARPP PIGAEVPDVT ATPARLLFFA PTRRAAPLEE MEAPAADAIM SPEEELDGYE PEPLGKRPAV LPLLELVGES GNNTSTDGSL PSTPPPAEEE EDELYRQSLE IISRYLREQA TGAKDTKPMG RSGATSRKAL ETLRRVGDGV QRNHETAFQG MLRKLDIKNE DDVKSLSRVM IHVFSDGVTN WGRIVTLISF GAFVAKHLKT INQESCIEPL AESITDVLVR TKRDWLVKQR GWDGFVEFFH VEDLEGG