Recombinant humaan MDL-1/CLEC5A-eiwit

CLEC5A, also known as C-type lectin domain family 5 member A isoform 1, is a member of the CTL/CTLD superfamily that have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. It functions as a cell attachment receptor for all four serotypes of Dengue virus as well as Japanese encephalitis virus. The binding of dengue virus to CLEC5A triggers signaling through the phosphylation of TYROBP, this interaction does not result in viral entry, but stimulates proinflammatory cytokine release. This protein acts as a positive regulator of osteoclastogenesis and a key regulator of synovial injury and bone erosion during autoimmune joint inflammation. Recombinant human CLEC5A protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

C-type lectin domain family 5 member A, C-type lectin domain family 5 member A isoform 1, CLEC5A, CLECSF5, MDL-1, MDL1, Macroprhage lectin 2

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

19.5 kDa (170aa)

CLEC5A, CLECSF5, C-type lectin domain family 5 member A, C-type lectin domain family 5 member A isoform 1, Kidney associated DCDC2 antisense RNA 1, Macroprhage lectin 2, MDL1, MDL-1, RU2, RU2 antisense gene protein, Ru2AS, ATGP3820-10 µg, ATGP3820-20 µg, ATGP3820-50 µg, ATGP3820-100 µg, ATGP3820-250 µg, ATGP3820-500 µg, ATGP3820-1 mg, ATGP3820-010, ATGP3820-020, ATGP3820-050, ATGP3820-100, ATGP3820-250, ATGP3820-500, ATGP3820-01M

Gonzalez-Dominguez E., et al, (2015) J Leukoc Biol. 98:453-466.; ; Chen ST., et al, (2017) Nat Commun. 8:299

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Immunology

NP_037384

Q9NY25

Human

Ala

ADLPQIFNKS NDGFTTTRSY GTVSQIFGSS SPSPNGFITT RSYGTVCPKD WEFYQARCFF LSTSESSWNE SRDFCKGKGS TLAIVNTPEK LKFLQDITDA EKYFIGLIYH REEKRWRWIN NSVFNGNVTN QNQNFNCATI GLTKTFDAAS CDISYRRICE KNAKHHHHHH