Recombinant humaan MED20-eiwit

MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors. Recombinant human MED20 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Mediator of RNA polymerase II transcription subunit 20, PRO0213, TRFP

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.6 kDa (235aa) confirmed by MALDI-TOF

MED20, Mediator of RNA polymerase II transcription subunit 20, PRO0213, TRFP, ATGP1855-10 µg, ATGP1855-20 µg, ATGP1855-50 µg, ATGP1855-100 µg, ATGP1855-250 µg, ATGP1855-500 µg, ATGP1855-1 mg, ATGP1855-10, ATGP1855-20, ATGP1855-50, ATGP1855-100, ATGP1855-250, ATGP1855-500, ATGP1855-1

Sato S, Tomomori-Sato C, et al. (2004) . Mol Cell. 14 (5) :685-91.; ; Zhang X, Krutchinsky A, et al. (2011) . Mol Cell. 19 (1) :89-100.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 50% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_004266

Q9H944

Human

MGVTCVS QMPVAEGKSV QQTVELLTRK LEMLGAEKQG TFCVDCETYH TAASTLGSQG QTGKLMYVMH NSEYPLSCFA LFENGPCLIA DTNFDVLMVK LKGFFQSAKA SKIETRGTRY QYCDFLVKVG TVTMGPSARG ISVEVEYGPC VVASDCWSLL LEFLQSFLGS HTPGAPAVFG NRHDAVYGPA DTMVQYMELF NKIRKQQQVP VAGIR