Recombinant humaan MED21-eiwit

MED21 is a member of the mediator complex subunit 21 family. The protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. Recombinant human MED21 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Mediator of RNA polymerase II transcription subunit 21, SRB7, SuRB7

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

18 kDa (167aa) confirmed by MALDI-TOF

MED21, Mediator of RNA polymerase II transcription subunit 21, SRB7, SuRB7, ATGP2118-10 µg, ATGP2118-20 µg, ATGP2118-50 µg, ATGP2118-100 µg, ATGP2118-250 µg, ATGP2118-500 µg, ATGP2118-1 mg, ATGP2118-10, ATGP2118-20, ATGP2118-50, ATGP2118-100, ATGP2118-250, ATGP2118-500, ATGP2118-1

Baumli, S., et al. (2005) J. Biol. Chem. 280 (18), 18171-18178; ; Maldonado, E., et al. (1996) Nature 381 (6577), 86-89

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_004255

Q13503

Human

MADRLTQ LQDAVNSLAD QFCNAIGVLQ QCGPPASFNN IQTAINKDQP ANPTEEYAQL FAALIARTAK DIDVLIDSLP SEESTAALQA ASLYKLEEEN HEAATCLEDV VYRGDMLLEK IQSALADIAQ SQLKTRSGTH SQSLPDS