Recombinant humaan MED27-eiwit

MED27 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e. g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Recombinant human MED27 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Mediator complex subunit 27, CRAP34, CRSP34, CRSP8, TRAP37

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

37.8 kDa (334aa)

MED27, Mediator complex subunit 27, CRAP34, CRSP34, CRSP8, TRAP37, ATGP2650-10 µg, ATGP2650-20 µg, ATGP2650-50 µg, ATGP2650-100 µg, ATGP2650-250 µg, ATGP2650-500 µg, ATGP2650-1 mg, ATGP2650-10, ATGP2650-20, ATGP2650-50, ATGP2650-100, ATGP2650-250, ATGP2650-500, ATGP2650-1

Malik S., et al. (2000) Mol. Cell. 5:753-760; ; Ryu S., et al. (1999) Nature. 397:446-450;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Epigenomics (Transcription & Translation)

NP_004260.2

Q6P2C8

Human

MADVINV SVNLEAFSQA ISAIQALRSS VSRVFDCLKD GMRNKETLEG REKAFIAHFQ DNLHSVNRDL NELERLSNLV GKPSENHPLH NSGLLSLDPV QDKTPLYSQL LQAYKWSNKL QYHAGLASGL LNQQSLKRSA NQMGVSAKRR PKAQPTTLVL PPQYVDDVIS RIDRMFPEMS IHLSRPNGTS AMLLVTLGKV LKVIVVMRSL FIDRTIVKGY NENVYTEDGK LDIWSKSNYQ VFQKVTDHAT TALLHYQLPQ MPDVVVRSFM TWLRSYIKLF QAPCQRCGKF LQDGLPPTWR DFRTLEAFHD TCRQ