Recombinant humaan MEK1-eiwit

MAP2K1, is a member of the bispecific protein kinase family, which acts as a mitogen-activated protein (MAP) kinase. It is expressed extensively and brain levels are very low. It is located upstream of the MAP kinase and stimulates the enzymatic activity of MAP kinases on a variety of extra-and-cellular signals. This protein, an essential component of the MAP kinase signaling pathway, is involved in many cellular processes such as proliferation, differentiation, transcriptional regulation and development. Recombinant human MAP2K1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Dual specificity mitogen-activated protein kinase kinase 1, MAP2K1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

44.5 kDa (402aa)

MEK1, Dual specificity mitogen-activated protein kinase kinase 1, MAP2K1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, ATGP3779-10 µg, ATGP3779-20 µg, ATGP3779-50 µg, ATGP3779-100 µg, ATGP3779-250 µg, ATGP3779-500 µg, ATGP3779-1 mg, ATGP3779-10, ATGP3779-20, ATGP3779-50, ATGP3779-100, ATGP3779-250, ATGP3779-500, ATGP3779-1

Hirata H., et al. (2012) Carcinogenesis. 33:41-48.; ; Rampoldi L., et al. (1998) Cytogenet Cell Genet. 78:301-303.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_002746.1

Q02750

Human

Ala

MPKKKPT PIQLNPAPDG SAVNGTSSAE TNLEALQKKL EELELDEQQR KRLEAFLTQK QKVGELKDDD FEKISELGAG NGGVVFKVSH KPSGLVMARK LIHLEIKPAI RNQIIRELQV LHECNSPYIV GFYGAFYSDG EISICMEHMD GGSLDQVLKK AGRIPEQILG KVSIAVIKGL TYLREKHKIM HRDVKPSNIL VNSRGEIKLC DFGVSGQLID SMANSFVGTR SYMSPERLQG THYSVQSDIW SMGLSLVEMA VGRYPIPPPD AKELELMFGC QVEGDAAETP PRPRTPGRPL SSYGMDSRPP MAIFELLDYI VNEPPPKLPS GVFSLEFQDF VNKCLIKNPA ERADLKQLMV HAFIKRSDAE EVDFAGWLCS TIGLNQPSTP THAAGV