Recombinant humaan MEMO1-eiwit

MEMO1 (Mediator of ErbB2-driven cell motility 1), also known as C2orf4 or NS5ATP7, belongs to the uPF0103 family. MEMO1 control cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. It is required for breast carcinoma cell migration, suggesting an important role in tumorigenesis. Also, it controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. Recombinant human MEMO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein MEMO1, C2orf4, CGI-27, MEMO, NS5ATP7

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

36.4 kDa (322aa) confirmed by MALDI-TOF

MEMO1, Protein MEMO1, C2orf4, CGI-27, MEMO, NS5ATP7, ATGP1619-10 µg, ATGP1619-20 µg, ATGP1619-50 µg, ATGP1619-100 µg, ATGP1619-250 µg, ATGP1619-500 µg, ATGP1619-1 mg, ATGP1619-10, ATGP1619-20, ATGP1619-50, ATGP1619-100, ATGP1619-250, ATGP1619-500, ATGP1619-1

Marone R., et al. (2004) Nat. Cell Biol. 6:515-522; ; Zaoui K., et al. (2010) Proc. Natl. Acad. Sci. u.S.A. 107:18517-18522

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 5mM DTT, 300 mM NaCl, 2mM EDTA

Signal Transduction

NP_057039

Q9Y316

Human

MGSSHHHHHH SSGLVPRGSH MGSHMMSNRV VCREASHAGS WYTASGPQLN AQLEGWLSQV QSTKRPARAI IAPHAGYTYC GSCAAHAYKQ VDPSITRRIF ILGPSHHVPL SRCALSSVDI YRTPLYDLRI DQKIYGELWK TGMFERMSLQ TDEDEHSIEM HLPYTAKAME SHKDEFTIIP VLVGALSESK EQEFGKLFSK YLADPSNLFV VSSDFCHWGQ RFRYSYYDES QGEIYRSIEH LDKMGMSIIE QLDPVSFSNY LKKYHNTICG RHPIGVLLNA ITELQKNGMN MSFSFLNYAQ SSQCRNWQDS SVSYAAGALT VH