Recombinant humaan MESDC1-eiwit

Mesoderm development candidate 1, also known as MESDC1, belongs to the MESD family. It is a 362 amino acid protein encoded by a gene that maps to human chromosome 15q25. 1. MESDC1 acts as a chaperone for low-density lipoprotein receptor (LDLR) proteins in the ER, and particularly associates with Wnt signaling pathway coreceptors LRP5 and LPR6) . MESDC1 is required for proper LRP5/6 folding and maturation to the cell surface, which plays a major role in Wnt signaling. Recombinant human MESDC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Mesoderm development candidate 1, MGC99595

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

40.1 kDa (385aa) confirmed by MALDI-TOF

MESDC1, Mesoderm development candidate 1, MGC99595, ATGP2657-10 µg, ATGP2657-20 µg, ATGP2657-50 µg, ATGP2657-100 µg, ATGP2657-250 µg, ATGP2657-500 µg, ATGP2657-1 mg, ATGP2657-10, ATGP2657-20, ATGP2657-50, ATGP2657-100, ATGP2657-250, ATGP2657-500, ATGP2657-1

Wines M E., et al. (2001) Genomics.72: 88-98.; ; Midia G S., et al. (2008) JAAPA. 21: 21-25.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Development

NP_072088

Q9H1K6

Human

MGSSHHHHHH SSGLVPRGSH MGSMASGSAG KPTGEAASPA PASAIGGASS QPRKRLVSVC DHCKGKMQLV ADLLLLSSEA RPVLFEGPAS SGAGAESFEQ CRDTIIARTK GLSILTHDVQ SQLNMGRFGE AGDSLVELGD LVVSLTECSA HAAYLAAVAT PGAQPAQPGL VDRYRVTRCR HEVEQGCAVL RATPLADMTP QLLLEVSQGL SRNLKFLTDA CALASDKSRD RFSREQFKLG VKCMSTSASA LLACVREVKV APSELARSRC ALFSGPLVQA VSALVGFATE PQFLGRAAAV SAEGKAVQTA ILGGAMSVVS ACVLLTQCLR DLAQHPDGGA KMSDHRERLR NSACAVSEGC TLLSQALRER SSPRTLPPVN SNSVN