Recombinant humaan mesotrypsine/PRSS3-eiwit

PRSS3, also known as trypsin-3, is a member of the trypsin family of serine proteases. It is specialized for the degradation of trypsin inhibitors and may be involved in defensin processing, including DEFA5. This protein is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Compared to PRSS1 and 2, one intriguing feature of PRSS3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. Recombinant human PRSS3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Brain trypsinogen, Mesotrypsin, Mesotrypsinogen, Serine protease 3, Serine protease 4, Trypsin-3, Trypsin III, Trypsin IV, PRSS4, TRY3, TRY4

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

25.3 kDa (233aa)

Trypsin3, PRSS3, MTG, PRSS4, T9, TRY3, TRY4, ATGP3220-10 µg, ATGP3220-20 µg, ATGP3220-50 µg, ATGP3220-100 µg, ATGP3220-250 µg, ATGP3220-500 µg, ATGP3220-1 mg, ATGP3220-10, ATGP3220-20, ATGP3220-50, ATGP3220-100, ATGP3220-250, ATGP3220-500, ATGP3220-1

Jiang G., et al. (2010) Gut 59 (11) :1535-1544.; ; Rosendahl J., et al. (2010) Pancreatology 10 (2-3) :243-249.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

NP_031369

P35030

Human

ADPIVGGYTC EENSLPYQVS LNSGSHFCGG SLISEQWVVS AAHCYKTRIQ VRLGEHNIKV LEGNEQFINA AKIIRHPKYN RDTLDNDIML IKLSSPAVIN ARVSTISLPT APPAAGTECL ISGWGNTLSF GADYPDELKC LDAPVLTQAE CKASYPGKIT NSMFCVGFLE GGKDSCQRDS GGPVVCNGQL QGVVSWGHGC AWKNRPGVYT KVYNYVDWIK DTIAANSHHH HHH