Recombinant humaan methionine Aminopeptidase 1/METAP1-eiwit

METAP1 (Methionine aminopeptidase 1) belongs to the peptidase M24A family. This protein removes the amino-terminal methionine from nascent proteins. It required for normal progression through the cell cycle. The active site of MetAP-1 contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. Recombinant human METAP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Methionyl aminopeptidase 1, MAP1A, MetAP1A

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.7 kDa (409aa) confirmed by MALDI-TOF

Methionine Aminopeptidase1, METAP1, methionyl aminopeptidase 1, MAP1A, MetAP1A, ATGP1509-10 µg, ATGP1509-20 µg, ATGP1509-50 µg, ATGP1509-100 µg, ATGP1509-250 µg, ATGP1509-500 µg, ATGP1509-1 mg, ATGP1509-10, ATGP1509-20, ATGP1509-50, ATGP1509-100, ATGP1509-250, ATGP1509-500, ATGP1509-1

Dummitt B, et al. (2002) Protein Pept Lett 9 (4) : 295-303.; ; Hu X., et al. (2006) Proc. Natl. Acad. Sci. u.S.A. 103:18148-18153.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 3mM DTT, 40% glycerol, 200 mM NaCl, 0.1mM PMSF

Metabolism

NP_055958

P53582

Human

MAAVETR VCETDGCSSE AKLQCPTCIK LGIQGSYFCS QECFKGSWAT HKLLHKKAKD EKAKREVSSW TVEGDINTDP WAGYRYTGKL RPHYPLMPTR PVPSYIQRPD YADHPLGMSE SEQALKGTSQ IKLLSSEDIE GMRLVCRLAR EVLDVAAGMI KPGVTTEEID HAVHLACIAR NCYPSPLNYY NFPKSCCTSV NEVICHGIPD RRPLQEGDIV NVDITLYRNG YHGDLNETFF VGEVDDGARK LVQTTYECLM QAIDAVKPGV RYRELGNIIQ KHAQANGFSV VRSYCGHGIH KLFHTAPNVP HYAKNKAVGV MKSGHVFTIE PMICEGGWQD ETWPDGWTAV TRDGKRSAQF EHTLLVTDTG CEILTRRLDS ARPHFMSQF