Recombinant humaan methionine-Aminopeptide 1D-eiwit

Methionine aminopeptidase 1D, mitochondrial precursor, also known as METAP1D, is a 335 amino acid mitochondrial protein that belongs to the peptidase M24A family. It is overexpressed in colon cancer cell lines, suggesting a role in tumorigenesis. METAP1D has also been found to remove methionine from the N-terminus of nascent proteins. METAP1D binds two cobalt ions per subunit and is encoded by a gene that maps to human chromosome 2q31. 1. Recombinant human METAP1D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Methionine aminopeptidase 1D mitochondrial precursor, Methionine aminopeptidase 1D, mitochondrial precursor, MAP1D, Metap1l

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

37.4 kDa (339aa) confirmed by MALDI-TOF

Methionine Aminopeptidase1D, Methionine aminopeptidase 1D mitochondrial precursor, Methionine aminopeptidase 1D, mitochondrial precursor, MAP1D, Metap1l, ATGP2467-10 µg, ATGP2467-20 µg, ATGP2467-50 µg, ATGP2467-100 µg, ATGP2467-250 µg, ATGP2467-500 µg, ATGP2467-1 mg, ATGP2467-10, ATGP2467-20, ATGP2467-50, ATGP2467-100, ATGP2467-250, ATGP2467-500, ATGP2467-1

Leszczyniecka M., et al. (2006) Oncogene. 25: 3471-3478.; ; Serero A., et al. (2003) J Biol Chem. 278: 52953-52963.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Immunology

NP_954697

Q6UB28

Human

SSPLNHI YLHKQSSSQQ RRNFFFRRQR DISHSIVLPA AVSSAHPVPK HIKKPDYVTT GIVPDWGDSI EVKNEDQIQG LHQACQLARH VLLLAGKSLK VDMTTEEIDA LVHREIISHN AYPSPLGYGG FPKSVCTSVN NVLCHGIPDS RPLQDGDIIN IDVTVYYNGY HGDTSETFLV GNVDECGKKL VEVARRCRDE AIAACRAGAP FSVIGNTISH ITHQNGFQVC PHFVGHGIGS YFHGHPEIWH HANDSDLPME EGMAFTIEPI ITEGSPEFKV LEDAWTVVSL DNQRSAQFEH TVLITSRGAQ ILTKLPHEA