Recombinant humaan METTL1-eiwit

METTL1 is known as tRNA (guanine-N (7) -) -methyltransferase which belongs to the methyltransferase superfamily. METTL1 shows high sequence similarity to yeast ORF YDL201w and has been shown to be inactivated by phosphorylation. This protein contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphylation. It catalyzes the formation of N (7) -methylguanine at position 46 (m7G46) in tRNA. Recombinant human METTL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Methyltransferase like 1, TRM8, YDL201w

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.6 kDa (296aa) confirmed by MALDI-TOF

METTL1, Methyltransferase like 1, TRM8, YDL201w, ATGP0869-10 µg, ATGP0869-20 µg, ATGP0869-50 µg, ATGP0869-100 µg, ATGP0869-250 µg, ATGP0869-500 µg, ATGP0869-1 mg, ATGP0869-10, ATGP0869-20, ATGP0869-50, ATGP0869-100, ATGP0869-250, ATGP0869-500, ATGP0869-1

Matsumoto K., et al. (2008) J. Biotechnol. 133: 453-460.; ; Alexandrov A., et al. (2002) RNA 8:1253-1266;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Enzymes & Proteases

NP_005362

Q9UBP6

Human

MAAETRNVAG AEAPPPQKRY YRQRAHSNPM ADHTLRYPVK PEEMDWSELY PEFFAPLTQN QSHDDPKDKK EKRAQAQVEF ADIGCGYGGL LVELSPLFPD TLILGLEIRV KVSDYVQDRI RALRAAPAGG FQNIACLRSN AMKHLPNFFY KGQLTKMFFL FPDPHFKRTK HKWRIISPTL LAEYAYVLRV GGLVYTITDV LELHDWMCTH FEEHPLFERV PLEDLSEDPV VGHLGTSTEE GKKVLRNGGK NFPAIFRRIQ DPVLQAVTSQ TSLPGH