Recombinant humaan MGAT2-eiwit

MGAT2 is a golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. Transcript variants with a spliced 5' uTR may exist, but their biological validity has not been determined. Recombinant human MGAT2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, Mannosyl alpha-1,6-glycoprotein beta-1,2-N-acetylglucosaminyltransferas, GlcNAc-T II, GNT-II, Mannoside acetylglucosaminyltransferase 2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II, CDG2A, CDGS2

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

50 kDa (439aa)

MGAT2, Mannosyl (alpha-16-) -glycoprotein beta-12-N-acetylglucosaminyltransferase, Mannosyl (alpha-1,6-) -glycoprotein beta-1,2-N-acetylglucosaminyltransferase, CDGS2, GLCNACTII, GNT-II, GNT2, ATGP2614-10 µg, ATGP2614-20 µg, ATGP2614-50 µg, ATGP2614-100 µg, ATGP2614-250 µg, ATGP2614-500 µg, ATGP2614-1 mg, ATGP2614-10, ATGP2614-20, ATGP2614-50, ATGP2614-100, ATGP2614-250, ATGP2614-500, ATGP2614-1

Tan J, Dunn J, et al. (1996) . Am J Hum Genet. 59 (4) :810-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Metabolism

NP_002399

Q10469

Human

MRQRKNEALA PPLLDAEPAR GAGGRGGDHP SVAVGIRRVS NVSAASLVPA VPQPEADNLT LRYRSLVYQL NFDQTLRNVD KAGTWAPREL VLVVQVHNRP EYLRLLLDSL RKAQGIDNVL VIFSHDFWST EINQLIAGVN FCPVLQVFFP FSIQLYPNEF PGSDPRDCPR DLPKNAALKL GCINAEYPDS FGHYREAKFS QTKHHWWWKL HFVWERVKIL RDYAGLILFL EEDHYLAPDF YHVFKKMWKL KQQECPECDV LSLGTYSASR SFYGMADKVD VKTWKSTEHN MGLALTRNAY QKLIECTDTF CTYDDYNWDW TLQYLTVSCL PKFWKVLVPQ IPRIFHAGDC GMHHKKTCRP STQSAQIESL LNNNKQYMFP ETLTISEKFT VVAISPPRKN GGWGDIRDHE LCKSYRRLQ