Recombinant humaan MGAT2-eiwit

MGAT2, also known as alpha-1, 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, is a single-pass type 2 membrane protein. It has the typical glycosyltransferase domains that composes a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. This protein catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans. Sometimes mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. Recombinant human MGAT2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, Mannosyl alpha-1,6-glycoprotein beta-1,2-N-acetylglucosaminyltransferas, GlcNAc-T II, GNT-II, Mannoside acetylglucosaminyltransferase 2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II, CDG2A, CDGS2

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

49.3 kDa (427aa)

MGAT2, Alpha-1, 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, MGAT2, CDG2A, CDGS2, GLCNACTII, GNT-II, GNT2, ATGP3770-10 µg, ATGP3770-20 µg, ATGP3770-50 µg, ATGP3770-100 µg, ATGP3770-250 µg, ATGP3770-500 µg, ATGP3770-1 mg, ATGP3770-10, ATGP3770-20, ATGP3770-50, ATGP3770-100, ATGP3770-250, ATGP3770-500, ATGP3770-1

Tan J., et al, (1996) Am. J. Hum. Genet. 59:810-817.; ; Maszczak-Seneczko D., et al, (2015) J. Biol. Chem. 290:15475-15486.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol, 0.15M NaCl, 1mM DTT

Enzymes & Proteases

NP_002399

Q10469

Human

Ala

ADPRQRKNEA LAPPLLDAEP ARGAGGRGGD HPSVAVGIRR VSNVSAASLV PAVPQPEADN LTLRYRSLVY QLNFDQTLRN VDKAGTWAPR ELVLVVQVHN RPEYLRLLLD SLRKAQGIDN VLVIFSHDFW STEINQLIAG VNFCPVLQVF FPFSIQLYPN EFPGSDPRDC PRDLPKNAAL KLGCINAEYP DSFGHYREAK FSQTKHHWWW KLHFVWERVK ILRDYAGLIL FLEEDHYLAP DFYHVFKKMW KLKQQECPEC DVLSLGTYSA SRSFYGMADK VDVKTWKSTE HNMGLALTRN AYQKLIECTD TFCTYDDYNW DWTLQYLTVS CLPKFWKVLV PQIPRIFHAG DCGMHHKKTC RPSTQSAQIE SLLNNNKQYM FPETLTISEK FTVVAISPPR KNGGWGDIRD HELCKSYRRL QHHHHHH