Recombinant humaan MICA-eiwit

MICA also known as MHC class I polypeptide-related sequence A. This Protein's Function seems to have no role in antigen presentation. It acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. It binding to KLRK1 leads to cell lysis. Recombinant human MICA, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Human MICA is coated at 10 µg/mL (100 ul/well) can bind Human NKG2D (CAT# ATGP3662) in a Functional ELISA assay.

MHC class I polypeptide-related sequence A, MHC class I polypeptide-related sequence A, PERB11.1, truncated

E.coli

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human NKG2D (CAT# ATGP3662)

32.7 kDa (283aa) confirmed by MALDI-TOF

MICA, MHC class I polypeptide-related sequence A, MHC class I polypeptide-related sequence A, PERB11.1, truncated, ATGP3053-10 µg, ATGP3053-20 µg, ATGP3053-50 µg, ATGP3053-100 µg, ATGP3053-250 µg, ATGP3053-500 µg, ATGP3053-1 mg, ATGP3053-10, ATGP3053-20, ATGP3053-50, ATGP3053-100, ATGP3053-250, ATGP3053-500, ATGP3053-1

Strausberg, R.L, et al. (2002) Proc. Natl. Acad. Sci. u.S.A. 99 (26), 16899-16903

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol

Immunology

AAH16929.1

Q29983

Human