Recombinant humaan Midkine-eiwit

Midkine, also known as Neurite Growth-promoting Factor 2, is a basic heparin-binding growth factor of low molecular weight, a member of the NEGF family whose founding member is pleiotrophin. This protein exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Midkine is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. Recombinant human Midkine protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

MDK, MK, NEGF2, midkine (neurite growth-promoting factor 2), FLJ27379, Midkine

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

15.7 kDa (144aa) confirmed by MALDI-TOF

Midkine, MDK, MK, NEGF2, midkine (neurite growth-promoting factor 2), FLJ27379, Midkine, ATGP0353-10 µg, ATGP0353-20 µg, ATGP0353-50 µg, ATGP0353-100 µg, ATGP0353-250 µg, ATGP0353-500 µg, ATGP0353-1 mg, ATGP0353-10, ATGP0353-20, ATGP0353-50, ATGP0353-100, ATGP0353-250, ATGP0353-500, ATGP0353-1

Mashima T., et al. (2009) . Oncogene. 28 (1) :9-19. ; ; Jin Z., et al. (2008) . Clin Cancer Res. 14 (16) :5033-42.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 2mM DTT, and 20% glycerol

Growth factors

NP_002382

P21741

Human

MGSSHHHHHH SSGLVPRGSH MVAKKKDKVK KGGPGSECAE WAWGPCTPSS KDCGVGFREG TCGAQTQRIR CRVPCNWKKE FGADCKYKFE NWGACDGGTG TKVRQGTLKK ARYNAQCQET IRVTKPCTPK TKAKAKAKKG KGKD