Recombinant humaan MIEN1-eiwit

MIEN1 belongs to the SelWTH family. This protein Increases cell migration by inducing filopodia formation at the leading edge of migrating cells and plays a role in regulation of apoptosis, possibly through control of CASP3. It also may be involved in a redox-related process. Recombinant human MIEN1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Migration and invasion enhancer 1, C17orf37, C35, ORB3, RDX12, XTP4

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

14.5 kDa (135aa) confirmed by MALDI-TOF

MIEN1, Migration and invasion enhancer 1, C17orf37, C35, ORB3, RDX12, XTP4, ATGP2271-10 µg, ATGP2271-20 µg, ATGP2271-50 µg, ATGP2271-100 µg, ATGP2271-250 µg, ATGP2271-500 µg, ATGP2271-1 mg, ATGP2271-10, ATGP2271-20, ATGP2271-50, ATGP2271-100, ATGP2271-250, ATGP2271-500, ATGP2271-1

Dasgupta, S., et al. (2011) J. Biol. Chem. 286 (29), 25935-25946; ; Katz, E., et al. (2011) Anal Cell Pathol (Amst) 34 (1-2), 35-48

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Cancer

NP_115715

Q9BRT3

Human

MGSSHHHHHH SSGLVPRGSH MGSMSGEPGQ TSVAPPPEEV EPGSGVRIVV EYCEPCGFEA TYLELASAVK EQYPGIEIES RLGGTGAFEI EINGQLVFSK LENGGFPYEK DLIEAIRRAS NGETLEKITN SRPPC