Recombinant humaan MINA-eiwit

MINA is an oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. This protein is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. It also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. MINA may play an important role in cell growth and survival. It may be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. Recombinant human MINA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

MYC induced nuclear antigen, FLJ14393, MDIG, MINA53, NO52

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

54.9 kDa (485aa)

MINA, MYC induced nuclear antigen, FLJ14393, MDIG, MINA53, NO52, ATGP2802-10 µg, ATGP2802-20 µg, ATGP2802-50 µg, ATGP2802-100 µg, ATGP2802-250 µg, ATGP2802-500 µg, ATGP2802-1 mg, ATGP2802-10, ATGP2802-20, ATGP2802-50, ATGP2802-100, ATGP2802-250, ATGP2802-500, ATGP2802-1

Strausberg, R.L., et al. (2002) Proc. Natl. Acad. Sci. u.S.A. 99 (26), 16899-16903

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

AAH14928

Q8IUF8

Human

MPKKAKPTGS GKEEGPAPCK QMKLEAAGGP SALNFDSPSS LFESLISPIK TETFFKEFWE QKPLLIQRDD PALATYYGSL FKLTDLKSLC SRGMYYGRDV NVCRCVNGKK KVLNKDGKAH FLQLRKDFDQ KRATIQFHQP QRFKDELWRI QEKLECYFGS LVGSNVYITP AGSQGLPPHY DDVEVFILQL EGEKHWRLYH PTVPLAREYS VEAEERIGRP VHEFMLKPGD LLYFPRGTIH QADTPAGLAH STHVTISTYQ NNSWGDFLLD TISGLVFDTA KEDVELRTGI PRQLLLQVES TTVATRRLSG FLRTLADRLE GTKELLSSDM KKDFIMHRLP PYSAGDGAEL STPGGKLPRL DSVVRLQFKD HIVLTVLPDQ DQSDETQEKM VYIYHSLKNS RETHMMGNEE ETEFHGLRFP LSHLDALKQI WNSPAISVKD LKLTTDEEKE SLVLSLWTEC LIQVV