Recombinant humaan MIS12-eiwit

Protein MIS12 homolog, also known as MIS12, is a component of the MIS12 complex, which is required for kinetochore formation during mitosis and normal chromosome alignment and segregation. The MIS12 complex consists of MIS12, DSN1, NSL1 and PMF-1. MIS12 is part of a network of complexes that provide microtubule attachment and generates pulling forces from depolymerization. Recombinant human MIS12 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Protein MIS12 homolog, hMis12, KNTC2AP, MTW1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.5 kDa (228aa)

MIS12, Protein MIS12 homolog, hMis12, KNTC2AP, MTW1, ATGP1928-10 µg, ATGP1928-20 µg, ATGP1928-50 µg, ATGP1928-100 µg, ATGP1928-250 µg, ATGP1928-500 µg, ATGP1928-1 mg, ATGP1928-10, ATGP1928-20, ATGP1928-50, ATGP1928-100, ATGP1928-250, ATGP1928-500, ATGP1928-1

Kline S L., et al. (2006) J Cell Biol. 173:9-17.; ; Goshima G., et al. (2003) J Cell Biol. 160:25-39.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 10% glycerol

Cell Cycle

NP_076944

Q9H081

Human

MSVDPMT YEAQFFGFTP QTCMLRIYIA FQDYLFEVMQ AVEQVILKKL DGIPDCDISP VQIRKCTEKF LCFMKGHFDN LFSKMEQLFL QLILRIPSNI LLPEDKCKET PYSEEDFQHL QKEIEQLQEK YKTELCTKQA LLAELEEQKI VQAKLKQTLT FFDELHNVGR DHGTSDFRES LVSLVQNSRK LQNIRDNVEK ESKRLKIS