Recombinant humaan MK3-eiwit

MAP2K3, also known as dual specificity mitogen-activated protein kinase kinase 3, is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Recombinant human MAP2K3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Dual specificity mitogen-activated protein kinase kinase 3, MAPKK3, MEK3, MKK3, PRKMK3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

38.3 kDa (338aa) confirmed by MALDI-TOF

MKK3, Dual specificity mitogen-activated protein kinase kinase 3, MAPKK3, MEK3, MKK3, PRKMK3, ATGP0999-10 µg, ATGP0999-20 µg, ATGP0999-50 µg, ATGP0999-100 µg, ATGP0999-250 µg, ATGP0999-500 µg, ATGP0999-1 mg, ATGP0999-10, ATGP0999-20, ATGP0999-50, ATGP0999-100, ATGP0999-250, ATGP0999-500, ATGP0999-1

Rampoldi L., et al. (1997) Cytogenet Cell Genet. 78 (3-4) :301-3.; ; Barr AJ., et al. (2002) Biochem Biophys Res Commun. 293 (1) :647-52.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Immunology

NP_002747.2

P46734

Human

MSKPPAPNPT PPRNLDSRTF ITIGDRNFEV EADDLVTISE LGRGAYGVVE KVRHAQSGTI MAVKRIRATV NSQEQKRLLM DLDINMRTVD CFYTVTFYGA LFREGDVWIC MELMDTSLDK FYRKVLDKNM TIPEDILGEI AVSIVRALEH LHSKLSVIHR DVKPSNVLIN KEGHVKMCDF GISGYLVDSV AKTMDAGCKP YMAPERINPE LNQKGYNVKS DVWSLGITMI EMAILRFPYE SWGTPFQQLK QVVEEPSPQL PADRFSPEFV DFTAQCLRKN PAERMSYLEL MEHPFFTLHK TKKTDIAAFV KEILGEDS