Recombinant humaan MLF1-eiwit

MLF1 (Myeloid leukemia factor 1) belongs to MLF family, and is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. This protein interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 normally functions in multi-potent progenitor cells, and its dysregulation may be somewhat responsible for leukemogenesis. Recombinant human MLF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Myeloid leukemia factor 1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

32.7 kDa (288aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

MLF1, Myeloid leukemia factor 1, ATGP0906-10 µg, ATGP0906-20 µg, ATGP0906-50 µg, ATGP0906-100 µg, ATGP0906-250 µg, ATGP0906-500 µg, ATGP0906-1 mg, ATGP0906-10, ATGP0906-20, ATGP0906-50, ATGP0906-100, ATGP0906-250, ATGP0906-500, ATGP0906-1

Yoneda-Kato N., et al. (2005) EMBO J. 24:1739-1749; ; Winteringham L.N., et al (2004) Oncogene 23: 5105-5109

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 5mM DTT, 200 mM NaCl

Cell Cycle

NP_071888

P58340

Human

MFRMLNSSFE DDPFFSESIL AHRENMRQMI RSFSEPFGRD LLSISDGRGR AHNRRGHNDG EDSLTHTDVS SFQTMDQMVS NMRNYMQKLE RNFGQLSVDP NGHSFCSSSV MTYSKIGDEP PKVFQASTQT RRAPGGIKET RKAMRDSDSG LEKMAIGHHI HDRAHVIKKS KNKKTGDEEV NQEFINMNES DAHAFDEEWQ SEVLKYKPGR HNLGNTRMRS VGHENPGSRE LKRREKPQQS PAIEHGRRSN VLGDKLHIKG SSVKSNKK