Recombinant humaan MMAB-eiwit

MMAB is a protein that catalyzes the final step in the conversion of vitamin B (12) into adenosylcobalamin (AdoCbl), a vitamin B12 containing coenzyme for methylmalonyl-CoA mutase (MCM) . Impaired MMAB activity leads to the inherited disorder vitamin B12 dependent methylmalonic aciduria linked to the cblB complementation group. Recombinant human MMAB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Methylmalonic aciduria (cobalamin deficiency) cblB type, ATR, ATP:cob (I) alamin adenosyltransferase, Methylmalonic aciduria (cobalamin deficiency) cblB type

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

26.3 kDa (239aa) confirmed by MALDI-TOF

MMAB, Methylmalonic aciduria (cobalamin deficiency) cblB type, ATR, ATP:cob (I) alamin adenosyltransferase, Methylmalonic aciduria (cobalamin deficiency) cblB type, ATGP0677-10 µg, ATGP0677-20 µg, ATGP0677-50 µg, ATGP0677-100 µg, ATGP0677-250 µg, ATGP0677-500 µg, ATGP0677-1 mg, ATGP0677-10, ATGP0677-20, ATGP0677-50, ATGP0677-100, ATGP0677-250, ATGP0677-500, ATGP0677-1

Gravel RA., et al. (2009) Mol Genet Metab. 98 (3) :278-84; ; Edwards AM., et al (2004) J Biol Chem. 279 (22) :23646-53.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Enzymes & Proteases

AAH05054

Q96EY8

Human

MQSRGPQGVE DGDRPQPSSK TPRIPKIYTK TGDKGFSSTF TGERRPKDDQ VFEAVGTTDE LSSAIGFALE LVTEKGHTFA EELQKIQCTL QDVGSALATP CSSAREAHLK YTTFKAGPIL ELEQWIDKYT SQLPPLTAFI LPSGGKISSA LHFCRAVCRR AERRVVPLVQ MGETDANVAK FLNRLSDYLF TLARYAAMKE GNQEKIYKKN DPSAESEGL