Recombinant humaan MMACHC-eiwit

The exact function of MMACHC is not known, however, its C-terminal region shows similarity to TonB, a bacterial protein involved in energy transduction for cobalamin (vitamin B12) uptake. Hence, it is postulated that this protein may have a role in the binding and intracellular trafficking of cobalamin. Mutations in this protein are associated with methylmalonic aciduria and homocystinuria type cblC. Recombinant human MMACHC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Methylmalonic aciduria and homocystinuria type C protein, RP11-291L19.3, cblC

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.3 kDa (306aa) confirmed by MALDI-TOF

MMACHC, Methylmalonic aciduria and homocystinuria type C protein, RP11-291L19.3, cblC, ATGP1574-10 ��g, ATGP1574-20 µg, ATGP1574-50 µg, ATGP1574-100 µg, ATGP1574-250 µg, ATGP1574-500 µg, ATGP1574-1 mg, ATGP1574-10, ATGP1574-20, ATGP1574-50, ATGP1574-100, ATGP1574-250, ATGP1574-500, ATGP1574-1

Lerner-Ellis JP., et al. (2006) Nat Genet. 38 (1) :93-100.; ; Tsai AC., et al. (2007) Am. J. Med. Genet. A 143A (20) : 2430-4.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Signal Transduction

NP_056321

Q9Y4U1

Human

MEPKVA ELKQKIEDTL CPFGFEVYPF QVAWYNELLP PAFHLPLPGP TLAFLVLSTP AMFDRALKPF LQSCHLRMLT DPVDQCVAYH LGRVRESLPE LQIEIIADYE VHPNRRPKIL AQTAAHVAGA AYYYQRQDVE ADPWGNQRIS GVCIHPRFGG WFAIRGVVLL PGIEVPDLPP RKPHDCVPTR ADRIALLEGF NFHWRDWTYR DAVTPQERYS EEQKAYFSTP PAQRLALLGL AQPSEKPSSP SPDLPFTTPA PKKPGNPSRA RSWLSPRVSP PASPGP