Recombinant humaan MMP-1-eiwit

MMP1, also known as interstitial collagenase isoform 1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. It is capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. It plays a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Recombinant human MMP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Interstitial collagenase isoform 1, MMP1, CLG, CLGN

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

53.1 kDa (460aa)

MMP1, Interstitial collagenase isoform 1, MMP1, CLG, CLGN, ATGP3439-10 µg, ATGP3439-20 µg, ATGP3439-50 µg, ATGP3439-100 µg, ATGP3439-250 µg, ATGP3439-500 µg, ATGP3439-1 mg, ATGP3439-10, ATGP3439-20, ATGP3439-50, ATGP3439-100, ATGP3439-250, ATGP3439-500, ATGP3439-1

Kumar JD., et al. (2015) Am J Physiol Gastrointest Liver Physiol. 309:G78-G86.; ; Yang H., et al. (2015) Biochemistry. 54:3631-3639.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM MES buffer (pH 5.5) containing 10 mM CaCl2, 100 mM NaCl, 0.05% Brij35, 30% glycerol

Adhesion & Movement

NP_002412

P03956

Human

His18

HSFPATLETQ EQDVDLVQKY LEKYYNLKND GRQVEKRRNS GPVVEKLKQM QEFFGLKVTG KPDAETLKVM KQPRCGVPDV AQFVLTEGNP RWEQTHLTYR IENYTPDLPR ADVDHAIEKA FQLWSNVTPL TFTKVSEGQA DIMISFVRGD HRDNSPFDGP GGNLAHAFQP GPGIGGDAHF DEDERWTNNF REYNLHRVAA HELGHSLGLS HSTDIGALMY PSYTFSGDVQ LAQDDIDGIQ AIYGRSQNPV QPIGPQTPKA CDSKLTFDAI TTIRGEVMFF KDRFYMRTNP FYPEVELNFI SVFWPQLPNG LEAAYEFADR DEVRFFKGNK YWAVQGQNVL HGYPKDIYSS FGFPRTVKHI DAALSEENTG KTYFFVANKY WRYDEYKRSM DPGYPKMIAH DFPGIGHKVD AVFMKDGFFY FFHGTRQYKF DPKTKRILTL QKANSWFNCR KNLEHHHHHH