Recombinant humaan MMS2/UBE2V2-eiwit

UBE2V2, also known as ubiquitin-conjugating enzyme E2 variant 2, constitutes a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. This protein also shares homology with ubiquitin-conjugating enzyme E2 variant 1 and yeast MMS2 gene product. It may be involved in the differentiation of monocytes and enterocytes. Recombinant human UBE2V2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 variant 2, DDVit 1, Enterocyte differentiation-associated factor 1, EDAF-1, Enterocyte differentiation-promoting factor 1, EDPF-1, MMS2 homolog, Vitamin D3-inducible protein, UEV2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

18.5 kDa (165aa) confirmed by MALDI-TOF

DDVit 1, DDVit1, DDVit-1, EDAF 1, EDAF1, EDAF-1, EDPF 1, EDPF1, EDPF-1, Enterocyte differentiation associated factor 1, Enterocyte differentiation promoting factor 1, Enterocyte differentiation-associated factor 1, Enterocyte differentiation-promoting factor 1, MMS2, MMS2 homolog, UBE2V2, Ubiquitin conjugating enzyme E2 variant 2, Ubiquitin-conjugating enzyme E2 variant 2, UEV 2, UEV2, UEV-2, Vitamin D3 inducible protein, Vitamin D3-inducible protein, ATGP0642-10 µg, ATGP0642-20 µg, ATGP0642-50 µg, ATGP0642-100 µg, ATGP0642-250 µg, ATGP0642-500 µg, ATGP0642-1 mg, ATGP0642-010, ATGP0642-020, ATGP0642-050, ATGP0642-100, ATGP0642-250, ATGP0642-500, ATGP0642-01M

Unk I., et al. (2008) Proc Natl Acad Sci u S A. 105 (10) :3768-73.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Ubiquitination

NP_003341

Q15819

Human

MAVSTGVKVP RNFRLLEELE EGQKGVGDGT VSWGLEDDED MTLTRWTGMI IGPPRTNYEN RIYSLKVECG PKYPEAPPSV RFVTKINMNG INNSSGMVDA RSIPVLAKWQ NSYSIKVVLQ ELRRLMMSKE NMKLPQPPEG QTYNN