Recombinant humaan MOBKL2B/MOB3B-eiwit

MOB3B shares similarity with the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. This gene is located on the opposite strand as the interferon kappa precursor (IFNK) gene. Recombinant human MOB3B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

MOB kinase activator 3B, C9orf35, MOB1D, MOBKL2B, Mob1 homolog 2b, Mps one binder kinase activator-like 2B, MOB kinase activator-like 2B, Monopolar spindle 1 binding MOB1 domain containing

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.9 kDa (239aa) confirmed by MALDI-TOF

MOB3B, MOB kinase activator 3B, C9orf35, MOB1D, MOBKL2B, ATGP2526-10 µg, ATGP2526-20 µg, ATGP2526-50 µg, ATGP2526-100 µg, ATGP2526-250 µg, ATGP2526-500 µg, ATGP2526-1 mg, ATGP2526-10, ATGP2526-20, ATGP2526-50, ATGP2526-100, ATGP2526-250, ATGP2526-500, ATGP2526-1

Akaogi K., et al (1994) . Biochem. Biophys. Res. Commun. 198:1046-1053; ; Oh Y., et a.l (1996) . J. Biol. Chem. 271:30322-30325;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Other

NP_079037

Q86TA1

Human

MGSSHHHHHH SSGLVPRGSH MGSMSIALKQ VFNKDKTFRP KRKFEPGTQR FELHKRAQAS LNSGVDLKAA VQLPSGEDQN DWVAVHVVDF FNRINLIYGT ICEFCTERTC PVMSGGPKYE YRWQDDLKYK KPTALPAPQY MNLLMDWIEV QINNEEIFPT CVGVPFPKNF LQICKKILCR LFRVFVHVYI HHFDRVIVMG AEAHVNTCYK HFYYFVTEMN LIDRKELEPL KEMTSRMCH