Recombinant humaan MOBKL3/MOB4-eiwit

Mps one binder kinase activator-like 3, also known as MOBKL3, belongs to the MOB1/Phocein family and is phosphorylated on serine residues. It is usually associated with membranes but can be present in the cytosol, where it behaves as a protein complex. It is the major partner of the striatin family members, which are scaffolding proteins involved in signaling and trafficking. Recombinant human MOBKL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

MOB-like protein phocein, 2C4D, Class II mMOB1, Mob1 homolog 3, Mob3, Mps one binder kinase activator-like 3, Preimplantation protein 3, MOB3, MOBKL3, PHOCN, PREI3, CGI-95

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

28.1 kDa (245aa) confirmed by MALDI-TOF

MOBKL3, Mps one binder kinase activator-like 3, MOB1, MOB3, PREI3, CGI-95, ATGP0833-10 µg, ATGP0833-20 µg, ATGP0833-50 µg, ATGP0833-100 µg, ATGP0833-250 µg, ATGP0833-500 µg, ATGP0833-1 mg, ATGP0833-10, ATGP0833-20, ATGP0833-50, ATGP0833-100, ATGP0833-250, ATGP0833-500, ATGP0833-1

Ponchon, L., et al. (2004) J. Mol. Biol. 337:167-182.; ; Baillat, G., et al. (2002) J Biol Chem. 277:18961-18966.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M Nacl, 5mM DTT, 10% glycerol

Metabolism

NP_056202

Q9Y3A3

Human

MGSSHHHHHH SSGLVPRGSH MVMAEGTAVL RRNRPGTKAQ DFYNWPDESF DEMDSTLAVQ QYIQQNIRAD CSNIDKILEP PEGQDEGVWK YEHLRQFCLE LNGLAVKLQS ECHPDTCTQM TATEQWIFLC AAHKTPKECP AIDYTRHTLD GAACLLNSNK YFPSRVSIKE SSVAKLGSVC RRIYRIFSHA YFHHRQIFDE YENETFLCHR FTKFVMKYNL MSKDNLIVPI LEEEVQNSVS GESEA