Recombinant humaan MOCS2-eiwit

MOCS2 is a heterotetrameric synthase composed of two small (MOCS2A) and two large (MOCS2B) subunits. The large and small subunits of molybdopterin synthase are both encoded from this gene by overlapping open reading frames. MOCS2 functions in the second step of the synthesis of molybdenum cofactor or molybdopterin (MPT) . It catalyzes the formation of MPT from precursor Z by incorporating a dithiolene functional group. Recombinant human MOCS2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molybdenum cofactor synthesis 2, molybdenum cofactor synthesis 2, MCBPE, MOCO1, MOCS2A, MOCS2B, MPTS

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25 kDa (224aa) confirmed by MALDI-TOF

MOCS2, molybdenum cofactor synthesis 2, molybdenum cofactor synthesis 2, MCBPE, MOCO1, MOCS2A, MOCS2B, MPTS, ATGP1712-10 µg, ATGP1712-20 µg, ATGP1712-50 µg, ATGP1712-100 µg, ATGP1712-250 µg, ATGP1712-500 µg, ATGP1712-1 mg, ATGP1712-10, ATGP1712-20, ATGP1712-50, ATGP1712-100, ATGP1712-250, ATGP1712-500, ATGP1712-1

Leimkuhler S., et al. (2005) Hum Genet. 117: 565-570; ; Reiss J., et al. (1999) Hum Genet . 64: 706-711.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol,1mM DTT

Metabolism

NP_004522

O96007

Human

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSSL EISSSCFSLE TKLPLSPPLV EDSAFEPSRK DMDEVEEKSK DVINFTAEKL SVDEVSQLVI SPLCGAISLF VGTTRNNFEG KKVISLEYEA YLPMAENEVR KICSDIRQKW PVKHIAVFHR LGLVPVSEAS IIIAVSSAHR AASLEAVSYA IDTLKAKVPI WKKEIYEESS TWKGNKECFW ASNS