Recombinant humaan monoglyceride Lipase/MGLL-eiwit

MGLL is membrane-associated member of the serine hydrolase superfamily. MGLL functions together with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides. It is most abundantly expressed in skeletal muscle and adipose tissue. Recombinant human MGLL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is >170 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C.

Monoglyceride lipase, Hu-K5, HuK5, MAGL, MGL, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

Specific activity is >170unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C.

36.4 kDa (333aa) confirmed by MALDI-TOF

Hu K5, HuK5, Hu-K5, Lysophospholipase homolog, Lysophospholipase-like, MAGL, MGL, MGLL, Monoglyceride Lipase, ATGP3430-10 µg, ATGP3430-20 µg, ATGP3430-50 µg, ATGP3430-100 µg, ATGP3430-250 µg, ATGP3430-500 µg, ATGP3430-1 mg, ATGP3430-010, ATGP3430-020, ATGP3430-050, ATGP3430-100, ATGP3430-250, ATGP3430-500, ATGP3430-01M

Cravatt BF., et al. (2010) Cell. 140 (1) :49-61.; ; Lambert DM., et al (2010) Chembiochem.11 (2) :218-27.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Metabolism

NP_009214

Q99685

Human

MGSSHHHHHH SSGLVPRGSH METGPEDPSS MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE LARMLMGLDL LVFAHDHVGH GQSEGERMVV SDFHVFVRDV LQHVDSMQKD YPGLPVFLLG HSMGGAIAIL TAAERPGHFA GMVLISPLVL ANPESATTFK VLAAKVLNLV LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFG IQLLNAVSRV ERALPKLTVP FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTN SVFHEINMWV SQRTATAGTA SPP