Recombinant humaan MP1/LAMTOR3-eiwit

MAPKSP1 (Mitogen-activated protein kinase scaffold protein 1) is a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Recombinant human MAPKSP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Mitogen-activated protein kinase scaffold protein 1, MAP2K1IP1, MAPBP, MP1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

15.8 kDa (144aa) confirmed by MALDI-TOF

MP1, MAPKSP1, Mitogen-activated protein kinase scaffold protein 1, MAP2K1IP1, MAPBP, MP1, ATGP0805-10 µg, ATGP0805-20 µg, ATGP0805-50 µg, ATGP0805-100 µg, ATGP0805-250 µg, ATGP0805-500 µg, ATGP0805-1 mg, ATGP0805-10, ATGP0805-20, ATGP0805-50, ATGP0805-100, ATGP0805-250, ATGP0805-500, ATGP0805-1

Wunderlich, et al. (2001) J. Cell Biol. (united States) 152 (4) : 765-76.; ; Schaeffer HJ, et al. (1998) Science 281 (5383) : 1668-71.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 100 mM NaCl

Signal Transduction

NP_068805

Q9UHA4

Human

MGSSHHHHHH SSGLVPRGSH MADDLKRFLY KKLPSVEGLH AIVVSDRDGV PVIKVANDNA PEHALRPGFL STFALATDQG SKLGLSKNKS IICYYNTYQV VQFNRLPLVV SFIASSSANT GLIVSLEKEL APLFEELRQV VEVS