Recombinant humaan MPG-eiwit

MPG (DNA-3-methyladenine glycosylase) belongs to the DNA glycosylase MPG family. This protein initiates base excision repair in DNA by removing a wide variety of alkylated, deaminated, and lipid peroxidation-induced purine adducts. Recombinant human MPG protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA-3-methyladenine glycosylase, AAG, ADPG, anpg, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.9 kDa (306aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

MPG, DNA-3-methyladenine glycosylase, AAG, ADPG, anpg, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16, ATGP1134-10 µg, ATGP1134-20 µg, ATGP1134-50 µg, ATGP1134-100 µg, ATGP1134-250 µg, ATGP1134-500 µg, ATGP1134-1 mg, ATGP1134-10, ATGP1134-20, ATGP1134-50, ATGP1134-100, ATGP1134-250, ATGP1134-500, ATGP1134-1

Adhikari S., et al. (2009) DNA Repair (Amst) . 8 (10) :1201-6.; ; Allan, J.M., et al. (1998) Cancer Res. 58: 3965-3973.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 200 mM NaCl, 1mM EDTA

Enzymes & Proteases

NP_002425.2

P29372

Human