Recombinant humaan MPI-eiwit

Mannose-6-phosphate isomerase, also known as MPI, belongs to the mannose-6-phosphate isomerase type 1 family, and is expressed in all tissues, more abundantly in heart, brain and skeletal muscle. Localized to the cytoplasm, MPI utilizes zinc as a cofactor and catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.

Mannose-6-phosphate isomerase, PMI, PMI1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

41.9 kDa (382aa)

MPI, Mannose-6-phosphate isomerase, PMI, PMI1, ATGP1192-10 µg, ATGP1192-20 µg, ATGP1192-50 µg, ATGP1192-100 µg, ATGP1192-250 µg, ATGP1192-500 µg, ATGP1192-1 mg, ATGP1192-10, ATGP1192-20, ATGP1192-50, ATGP1192-100, ATGP1192-250, ATGP1192-500, ATGP1192-1

Schollen E., et al. (2000) Hum Mutat. 16:247-252.; ; Proudfoot A E., et al. (1996) Biochem J. 318:437-442.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 5% glycerol

Metabolism

AAH17351

P34949

Human

MGSSHHHHHH SSGLVPRGSH MAAPRVFPLS CAVQQYAWGK MGSNSEVARL LASSDPLAQI AEDKPYAELW MGTHPRGDAK ILDNRISQKT LSQWIAENQD SLGSKVKDTF NGNLPFLFKV LSVETPLSIQ AHPNKELAEK LHLQAPQHYP DANHKPEMAI ALTPFQGLCG FRPVEEIVTF LKTAAGNNME DIFGELLLQL HQQYPGDIGC FAIYFLNLLT LKPGEAMFLE ANVPHAYLKG DCVECMACSD NTVRAGLTPK FIDVPTLCEM LSYTPSSSKD RLFLPTRSQE DPYLSIYDPP VPDFTIMKTE VPGSVTEYKV LALDSASILL MVQGTVIAST PTTQTPIPLQ RGGVLFIGAN ESVSLKLTEP KDLLIFRACC LL