Recombinant humaan MPST-eiwit

MPST catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDu) . Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. Recombinant human MPST protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

3-mercaptopyruvate sulfurtransferase, MST, TST2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.7 kDa (321aa) confirmed by MALDI-TOF

MPST, 3-mercaptopyruvate sulfurtransferase, MST, TST2, ATGP1860-10 µg, ATGP1860-20 µg, ATGP1860-50 µg, ATGP1860-100 µg, ATGP1860-250 µg, ATGP1860-500 µg, ATGP1860-1 mg, ATGP1860-10, ATGP1860-20, ATGP1860-50, ATGP1860-100, ATGP1860-250, ATGP1860-500, ATGP1860-1

Billaut-Laden I, Rat E, et al. (2006) . Toxicol Lett. 165 (2) :101-11.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Metabolism

NP_001013454

P25325

Human

MASPQL CRALVSAQWV AEALRAPRAG QPLQLLDASW YLPKLGRDAR REFEERHIPG AAFFDIDQCS DRTSPYDHML PGAEHFAEYA GRLGVGAATH VVIYDASDQG LYSAPRVWWM FRAFGHHAVS LLDGGLRHWL RQNLPLSSGK SQPAPAEFRA QLDPAFIKTY EDIKENLESR RFQVVDSRAT GRFRGTEPEP RDGIEPGHIP GTVNIPFTDF LSQEGLEKSP EEIRHLFQEK KVDLSKPLVA TCGSGVTACH VALGAYLCGK PDVPIYDGSW VEWYMRARPE DVISEGRGKT H