Recombinant humaan MPZL1-eiwit

MPZL1 is a cell surface receptor, which is involved in signal transduction processes. This protein recruits PTPN11/SHP-2 to the cell membrane and is a putative substrate of PTPN11/SHP-2. It is a major receptor for concanavalin-A (ConA) and is involved in cellular signaling induced by ConA, which probably includes Src family tyrosine-protein kinases. Isoform 3 seems to have a dominant negative role; it blocks tyrosine phosphorylation of MPZL1 induced by ConA. Isoform 1, but not isoform 2 and isoform 3, may be involved in regulation of integrin-mediated cell motility. Recombinant human MPZL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Myelin protein zero-like protein 1 isoform b, MPZL1b, PZR, PZR1b, PZRa, PZRb

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

16.1 kDa (148aa) confirmed by MALDI-TOF

MPZL1, Myelin protein zero-like protein 1 isoform b, MPZL1b, PZR, PZR1b, PZRa, PZRb, ATGP2225-10 µg, ATGP2225-20 µg, ATGP2225-50 µg, ATGP2225-100 µg, ATGP2225-250 µg, ATGP2225-500 µg, ATGP2225-1 mg, ATGP2225-10, ATGP2225-20, ATGP2225-50, ATGP2225-100, ATGP2225-250, ATGP2225-500, ATGP2225-1

Zhao ZJ., et al. (1998) . J Biol Chem 273 (45) : 29367-72.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_078845

O95297

Human

LEVYTPK EIFVANGTQG KLTCKFKSTS TTGGLTSVSW SFQPEGADTT VSFFHYSQGQ VYLGNYPPFK DRISWAGDLD KKDASINIEN MQFIHNGTYI CDVKNPPDIV VQPGHIRLYV VEKENLPV